Journal: Proteomes

Article Title: Cellular Responses of Maize Roots to Long-Term Cadmium Exposure: Adjustments of Class III Peroxidases, Plasma Membrane and Tonoplast Sub-Proteomes

doi: 10.3390/proteomes14010011

Figure Lengend Snippet: Partial purification of soluble guaiacol peroxidases by chromatofocusing. Samples were applied onto a Mono P column and eluted by a self-generating pH gradient. ( a ) Guaiacol peroxidase activities of control; ( b ) guaiacol peroxidase activities of Cd 2+ sample. Shown were typical elution profiles of control ( n = 3) and Cd 2+ samples ( n = 4) with guaiacol peroxidase activity (Prx, —■— ), protein absorbance ( - - - ) and pH gradient ( — ). Peak fractions were numbered according to pIs. Replicates are shown in .

Article Snippet: Proteins were partially purified by chromatofocusing on a Mono P column (HR 5/20, Amersham Pharmacia Biotech, Freiburg, Germany) using an HPLC-System (ÄKTA, Amersham Pharmacia Biotech, Freiburg, Germany) with 10 mL super-loop, UV cell, conductivity and pH electrodes.

Techniques: Purification, Control, Activity Assay